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Advances in Applied and Pharmaceutical Sciences Journal 

ISSN 2457-0842

Abstract


Volume 1 Issue 1, July-August 2017, Pages:24-32


Authors: Syed Ayaz Ali, Uma Bhandari , K K Pillai
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Abstract: The purpose of this study was to investigate protective effect of tempol as cardioprotective and antioxidant, on damage caused by doxorubicin (DOX). Wistar albino rats were used in this experiment. Doxorubicin was administered intraperitoneally in six equal injections (each containing 2.5 mg/kg doxorubicin at 48 h interval) to a total cumulative dose of 15 mg/kg over a period of 2 weeks to produce cardiotoxicity. Tempol was administered as pretreatment and post-treatment. The biochemical parameters such as tissue glutathione (GSH), malondialdehyde (MDA), lactate dehydrogenase (LDH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-s-transferase (GST), and glucose-6-phosphate dehydrogenase (G6PD) were monitored after 30 days. Pre-treatment and Post treatment with tempol significantly protected the myocardium from the toxic effects of doxorubicin, by increasing the levels of GSH and reducing the levels of antioxidant enzymes such as CAT, SOD, GPx, GR, GST, and G6PD towards normal and decreased the increased levels of MDA and LDH. It has also reduced the severity of cellular damage of the myocardium. The restoration of the endogenous antioxidant system clearly depicts that tempol has produced its protective effect by scavenging the reactive oxygen species (ROS). Our study shows that pretreatment with tempol as cardioprotective and antioxidant is more beneficial as compared to post treatment against DOX-induced cardiac toxicity
Cite this article: S A Ali, U Bhandari , K K Pillai“ Tempol Ameliorates Oxidative Stress, Apoptosis in Doxorubicin Induced Cardiotoxicity“Advances in Applied and Pharmaceutical Sciences Journal 2017, 1 (1);24-32